Isolamento e caracterização bioquímica e genética de bactérias ácidas do gênero Acetobacter a partir de três amostras de kombucha cultivadas no Estado de Goiás

Abstract

Kombucha is considered a probiotic, so it is widely used in folk medicine worldwide, originating in China. A structure of firm and gelatinous consistency is formed in the upper part, called mother of kombucha. In this structure are found associated microorganisms, with predominance of the genus Acetobacter acid metabolism. Thus this work consisted of the isolation of acid bacteria in three different kombucha mother matrices (M1, M2 and M3). Fifty-four bacteria were isolated and biochemically characterized by plaque growth test with 12 carbon sources and the presence of enzymes (proteases, cellulase and amylases) as well as growth in AAM, LGI-P and MacConkey selective media. By growth in AAM medium it is possible to indicate that all isolates are acid bacteria. Already the presence of halo in GYC medium was observed in 35 isolates indicating that they may be of the family Acetobacteriaceae, but other tests are needed. The dendrogram showed a tendency to group the isolates according to the isolation sample with 65% similarity. The most diverse sample was considered M1and M2 corroborating that there is a variation in the composition of bacteria depending on the source of kombucha mother and presence of acid bacteria. This study is preliminary and the isolated bacteria present in kombucha cultivated in the Cerrado will be used in other studies to evaluate the effect on the chemical composition of kombucha.